HTML Report

Concept


No successful marker extractions can be achieved without successful assemblies. Even though this assemble module offers presets tuned for different data types, it is recommendable to repeat this step some times with different parameters to find optimal settings for your own data.

Captus assists you in this tedious process by automatically generating a useful report for assembly evaluation.
Just open captus-assembly_assemble.report.html with your browser (internet connection required) to get general assembly statistics across all your samples!

Tip

Contents


1. Summary Table

This table shows the general assembly statistics for each sample.

Features:

  • Switch the Sort by dropdown to re-sort the table by any column value.
  • Cells are color-coded according to value (high = green; low = pink).

Columna Description Unit
Sample Sample name -
Total Length Passed Cumulative length of all contigs passed GC content filter bp
Total Length Removed Cumulative length of all contigs removed by GC content filter
(shown only when --max_contig_gc argument is enabled)
bp
Number of Contigs Passed Number of contigs passed GC content filter -
Number of Contigs Removed Number of contigs removed by GC content filter
(shown only when --max_contig_gc argument is enabled)
-
*Mean Length Mean contig length bp
*Contig N50 Weighted average of contig lengths that 50% of total assembly length consists of contigs over this length bp
*Longest Contig Longest contig length bp
*Shortest Contig Shortest contig length bp
*≥1 kbp Contigs Percentage of contigs over 1 kbp in total number of contigs %
GC Content Passed Mean GC content of contigs passed GC content filter %
GC Content Removed Mean GC content of contigs removed by GC content filter
(shown only when --max_contig_gc argument is enabled)
%
*Mean Depth Mean contig depth x

a Statistics denoted by * are calculated only from contigs passed GC content filter.


2. Visual Stats

This plot shows the statistics shown in the table above in addition to more detailed statistics as a bar graph.

Features:

  • Switch the dropdown at the y-axis to switch the variable to show.
  • Click on the legend to toggle hide/show of each data series.
Variablea Description Unit
Total Length Cumulative length of all contigs bp
Number of Contigs Number of contigs -
Mean Length Mean contig length bp
*Median Length Median contig length bp
*Contig N50 Weighted average of contig lengths that 50% of total assembly length consists of contigs over this length bp
*Longest Contig Longest contig length bp
*Shortest Contig Shortest contig length bp
*Contig Breakdown by Length Percentage of contigs over 1, 2, 5, and 10 kbp in total number of contigs %
*Length Breakdown by Contig Length Percentage of contigs over 1, 2, 5, and 10 kbp in total length of contigs %
GC Content Mean GC content of contigs %
*Mean Depth Mean contig depth x
*Contig Breakdown by Depth Percentage of contigs with over 1, 2, 5, and 10 x depth in total number of contigs %

a Statistics denoted by * are calculated only from contigs passed GC content filter.


Created by Gentaro Shigita (11.08.2021)
Last modified by Gentaro Shigita (17.10.2022)